NASC Stock Detail Page
NASC ID: N9561
Name: PtGFP_kn
ABRC stock number: CS9561
Description: Pt-GFP is a pH-reporter protein when used ratiometrically in the double excitation mode. It has a broad pH-responsiveness and a good acid stability.Arabidopsis lines PtGFP_Kn and PtGFP6-3 can be used to monitor cytoplasmic pH in a wide range of applications. PtGFP_kn and PtGFP6-3 represent two expression lines from two different floral dip experiments. Hence the T-DNA from the Agrobacs carrying the PtGFP gene has been inserted in different (as yet not identified) locations of the Arabidopsis genome, leading to different expression levels (kn appears a bit brighter than 6-3).
Donation Date: 2006-03-31
| Donated by: | Christian-Albrechts-Universität zu Kiel Christoph Plieth |
Donor Number: PtGFP_kn
Stock type: individual line
Material type: seed
Comment: related stock PtGFP6-3 . Further information is found at: http://www.nanolight.com/nanofluor.htm
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Single marker
Background: Col-0 ( N1092 )
Marker used: green fluorescent protein from Ptilosarcus gurneyi (seapen)
Generation: F2
Segregation: yes
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Phenotype
These lines express green fluorescent protein from the seapen Ptilosarcus gurneyi (Pt-GFP) constitutively in the cytoplasm under control of a single 35S-CaMV-promoter.Cells fluoresce green when exited with blue light.There are no other differences in phenotype neither in appearance and growth nor in development and reproduction when compared to Col-0 wild type.
References
Schulte, A., et al. 2006. A novel pH indicator for expression in plants. Plant methods 2:7.PubMed ID: 16600023.