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NASC

The European Arabidopsis Stock Centre

Wave lines DNA

Donated by

  • Chris Somerville Department of Plant Biology, The Carnegie Institution of Washington

Click here to view all 94 of these lines.

Description

Wave lines DNA

This collection has wave lines distributed as DNA clones, for wave lines distributed as seed stock go to Wave lines Seed.
Wave lines, a set of distinct subcellular markers with spectrally distinct, fluorescent fusion constructs- provided as DNA clones and vectors. The set provides an easy way to screen for distinct subcellular localization patterns and provides stable, non-toxic expression in transgenic plants. The constructs are under pUBQ10 promoter. The pNIGEL recombination vectors on which these constructs are based allow the generation of fusion constructs by CRE-lox recombination, using the pUNI ORF collection of the SSP consortium.

GenBank numbers for the pNIGELs

  • pNIGEL 07 : FJ493465
  • pNIGEL 17 : FJ493466
  • pNIGEL 18 : FJ493467
  • pNIGEL 19 : FJ530950
  • pNIGEL 09 : FJ524330
  • pNIGEL 11 : FJ524331
  • pNIGEL 13 : FJ524332
  • pNIGEL 15 : FJ524333
  • pNIGEL 16 : FJ524334

Nomenclature

The final marker constructs are available in four colors Blue (mCerulean), Bluegreen (mTFP1), Yellow (YFP) and Red (mCherry). It means there are 21 different genes, available in 4 colors: 84 clones in total. The nomenclature of the constructs is, for example: Wave 131C, Wave 131T, Wave 131Y or Wave 131R, the last letter indicating the different fluorophores.

Growth requirements in bacteria

  • For bacterial selection of stocks N781520 to N781603, use a final concentration of 50µg/ml Kanamycin.
  • For bacterial selection of pNIGEL recombination vectors (N781604 to N781612), use a final concentration of 100µg/ml Ampicillin.

Distribution

  • This collection contains DNA clones and vectors, all distributed as bacterial stabs
  • These stocks can all be ordered individually or as a complete set (all 84 clones + vectors) N781613

Individuals producing these lines

  • Niko Geldner, Valérie Dénervaud Tendon, York-Dieter Stierhof, Ulrike Mayer, Derek L. Hyman, Joanne Chory

Compliment slip and Plate Layout for the wave plate N781613

Compliment Slip and info

Plate layout for Wave plate

Related links

References

  • Geldner N, Dénervaud-Tendon V, Hyman DL, Mayer U, Stierhof YD, Chory J. Rapid, combinatorial analysis of membrane compartments in intact plants with a multi-color marker set. Plant J. 2009 Feb 26. PMID: 19309456