GABI-Kat
Donated by
- Bernd Weisshaar Centre for Genome Research, Faculty of Biology, Universitat Bielefeld
- Ji Hoon Ahn Graduate School of Biotechnology, Korea University
Description
GABI-Kat: generation of Flanking Sequence Tags (FSTs) from T-DNA mutagenised A. thaliana plants (accession Columbia)
GABI-Kat was initiated at the Max-Planck-Institute of Plant Breeding Research and aims to generate at least 80000 T-DNA transformants (sequenced transformant available at NASC as a segregating set) for the accession Columbia (Col-0). Seeds of single lines (T2 seeds from T1 plants) were harvested, and genomic DNA from T1 plants was extracted to produce FSTs from their insertion sites by a linker-ligation-PCR based approach. The FST data were then mapped to the genome and the results have been made available both via the internet at the GABI-Kat site, and as a bulk download.
GABI-Kat lines available to order at NASC
The GABI-Kat lines are now available via NASC at standard user fees (standard fee per line and £10 per set) to the international research community. GABI-Kat lines that have been confirmed will be transferred to NASC as sets of T3 seed harvested from a segregating family of single T2 plants. It is recommended that the set is ordered as each line within a set may be segregating for the insert. This will allow direct user access to potentially homozygous material for a given insertion line.
Explanation of line numbering system
Each set of T3 seeds has been produced from their respective GK-****** parental line. This parental line number is also the name given to the set. Each set and individual line also has its own NASC number. For example, the table below shows the set GK-067H03 and the individual lines that make up this set.
If you order a set you will receive all individual lines that make up the set. So in the example below if you order the set you will receive 12 individual lines for the price of £10.
| Name | NASC code | Set/Individual line | Price £ |
|---|---|---|---|
| GK-067H03 | N406423 | Set | 10 |
| GK-067H03.01 | N300001 | Ind. line | 3.50 |
| GK-067H03.02 | N300002 | Ind. line | 3.50 |
| GK-067H03.03 | N300003 | Ind. line | 3.50 |
| GK-067H03.04 | N300004 | Ind. line | 3.50 |
| GK-067H03.05 | N300005 | Ind. line | 3.50 |
| GK-067H03.06 | N300006 | Ind. line | 3.50 |
| GK-067H03.07 | N300007 | Ind. line | 3.50 |
| GK-067H03.08 | N300008 | Ind. line | 3.50 |
| GK-067H03.09 | N300009 | Ind. line | 3.50 |
| GK-067H03.10 | N300010 | Ind. line | 3.50 |
| GK-067H03.11 | N300011 | Ind. line | 3.50 |
| GK-067H03.12 | N300012 | Ind. line | 3.50 |
Segregation counts
Three numbers are counted from the selection plates:
For example - 100:92:75
total: total number of seeds on the plate.
germinated: number of seeds that show signs of a root tip.
resistant: seedlings that grow on the selection plate.
Selection was done using 5.25 mg/L sulfadiazine where wild type plants bleached and did not generate secondary leaves.
Order
Search the NASC catalogue for the GABI-Kat name e.g. "GK-067H03" (including the quotation marks). This will display the set and individual lines produced from the parental line.