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NASC

The European Arabidopsis Stock Centre

Sundaresan heat shock

Donated by

  • Venkatesan Sundaresan Department of Plant Biology, College of Biological Sciences, University of California, Davis
  • Bindu Nishal Department of Plant Biology, College of Biological Sciences, University of California, Davis
  • Titima Tantikanjana Institute of Molecular Agrobiology, National University of Singapore

Click here to view all 39 of these lines.

Description

Sundaresan heat shock lines

Lines generated by the system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis. The Ac and Ds elements were placed in the same T-DNA construct and a heat shock-inducible transposase fusion was utilized to control the levels of transposase gene expression, generating a transposition that can be subsequently stabilized without requiring crossing or segregation. These were mapped by thermal asymmetric interlaced-PCR. Lines can be used as potential launch pad for heat shock mutagenesis; Kanamycin resistant (50 mg/L).

References

  • Nishal, B., Tantikanjana, T. & Sundaresan, V. 2005. An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis. Plant physiology 137(1):3-12.PubMed ID: 15644463.