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The European Arabidopsis Stock Centre

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Search result for '105 '. Viewing records 101 to 150 of 304 hits.



N70550 Name: JYB447.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE49(At3g05150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak expression in cortex starting elongation zone (1/5; 5DAG); GFP from 4.5% sucrose growth is much weaker than 1% (1/4 ;5DAG; 4.5% sucrose - 2/4; 5DAG; 1% sucrose).
N70551 Name: JYB448.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE49(At3g05150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak expression in cortex starting elongation zone (3/8; 5DAG).
N70552 Name: JYB449.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE49(At3g05150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak expression in cortex starting elongation zone (3/5; 5DAG), possibly also CE initial and up; very weak cortex expression from elongation zone to maturation zone (2/4; 4.5% sucrose; 5DAG - 2/5; 1% sucrose 5DAG).
N70553 Name: JYB450.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE49(At3g05150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak to stronger expression in cortex starting elongation zone (5/5; 5DAG), possibly columella.
N70554 Name: JYB460.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS27(AT1G68130)::cS27:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the pericycle starting from early elongation zone up to maturation zone (7/7; 7DAG).
N70555 Name: JYB461.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS27(AT1G68130)::cS27:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the pericycle starting from late meristimatic zone (only in the pericycle of xylem pole up to early maturation zone) very weak GFP in endodermis and cortex from elongation zone(4/5), also very strong GFP in endodermis and cortex, possibly protein is moving (2/5; 5DAG).
N70556 Name: JYB462.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS27(AT1G68130)::cS27:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the pericycle starting from early elongation zone up to maturation zone (5/7), rarely found in cortex and endodermis (2/7; 5DAG).
N70557 Name: JYB470.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on plates and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: very weak signal in the stele of the meristematic zone (2/8; 6DAG).
N70558 Name: JYB470.3 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on plates and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: very weak signal in the stele of the meristematic zone (3/7; 5DAG).
N70559 Name: JYB470.4 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: very weak signal in the stele of the meristematic zone (5/8; 5DAG).
N70560 Name: JYB471.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on plates and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: very weak signal in the stele of the meristematic zone (2/7; 5DAG).
N70561 Name: JYB478.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong expression in xylem starting after elongation, before root hair (5/6; 7DAG).
N70562 Name: JYB478.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong expression in xylem starting after elongation, before root hair (3/6; 7DAG).
N70563 Name: JYB479.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong expression in xylem starting after elongation, before root hair (5/5; 7DAG).
N70564 Name: JYB480.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong expression in xylem starting after elongation, before root hair (4/6; 7DAG).
N70565 Name: JYB482.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::cS18:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in protoxylem of elongation zone and metaxylem in maturation zone (5/5; 6DAG).
N70566 Name: JYB486.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::cS18:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in protoxylem of elongation zone and metaxylem in maturation zone (4/5; 6DAG).
N70567 Name: JYB486.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS18(AT5G12870)::cS18:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in protoxylem of elongation zone and metaxylem in maturation zone (5/5; 6DAG).
N70568 Name: JYB499.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS17(AT2G22850)::cS17:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: very spotty GFP in stele (6; 6DAG).
N70569 Name: JYB506.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS21(AT3G49760)::cS21:GFP(-G):NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: spotty GFP in stele (5DAG).
N70570 Name: JYB507.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS21(AT3G49760)::cS21:GFP(-G):NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: spotty GFP in stele (5DAG).
N70571 Name: JYB512.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS19(AT4G37940)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak expression in columella, consistently in the procambium from meristematic zone to elongation zone (3/8; 4DAG); possibly in columella and xylem (5/5 4% sucrose 4DAG).
N70572 Name: JYB514.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS19(AT4G37940)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in columella, consistently in the procambium from meristematic zone to elongation zone (2/6; 4DAG).
N70573 Name: JYB516.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS19(AT4G37940)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in columella, lateral root cap, and stele (not clear if it is xylem or phloem) in meristematic zone and early elongation zone (3/6; 5DAG), (1/6; 4DAG) - possibly in more mature roots; possibly in columella and xylem (3/4; 4% sucrose; 4DAG).
N70574 Name: JYB521.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS21(AT3G49760)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: expression in the pericycle from late meristematic zone up to maturation zone on the phloem pole (4/6; 5DAG).
N70575 Name: JYB522.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS21(AT3G49760)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP expression in the pericycle of phloem pole on late meristematic zone upto maturation zone (3/7; 4DAG) (3/3; 5DAG).
N70576 Name: JYB551.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE40(AT5G24380)::ER-GFP:NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis, xylem from late maturation zone (6/6; 5DAG).
N70577 Name: JYB552.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE40(AT5G24380)::ER-GFP:NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis, xylem from late maturation zone (4/6; 5DAG).
N70578 Name: JYB554.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE40(AT5G24380)::ER-GFP:NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) lacking GFP marker expression in roots.
N70579 Name: JYB557.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS29(AT2G37590)::cS29:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: possible expression in the cells that will form phloem in the meristematic zone; no GFP expression in the elongation zone (5/5; 5DAG).
N70580 Name: JYB557.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS29(AT2G37590)::cS29:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: possible expression in the cells that will form phloem in the meristematic zone (7/7; 5DAG), one line showed very weak GFP in elongating phloem.
N70581 Name: JYB558.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS29(AT2G37590)::cS29:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: possible expression in the cells that will form phloem in the meristematic zone; no GFP expression in the elongation zone (3/4; 5DAG).
N70582 Name: JYB563.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE47(At2g37950)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis at start of long root hair (5/6; 6DAG).
N70583 Name: JYB565.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE47(At2g37950)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis at start of long root hair(5/6; 6DAG).
N70584 Name: JYB567.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE47(At2g37950)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis at start of long root hair (3/6; 6DAG).
N70585 Name: JYB567.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE47(At2g37950)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis at start of long root hair (5/6; 6DAG).
N70586 Name: JYB568.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pE47(At2g37950)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis at start of long root hair (3/5; 6DAG); expression starts later than lines CS70651, CS70652 and CS70653.
N70587 Name: JYB569.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS11(AT2G34710)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, stele, endodermis cortex in meristematic zone but inconsistent in endodermis/cortex (4/5; 4DAG).
N70588 Name: JYB570.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS11(AT2G34710)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, stele, endodermis cortex in meristematic zone stele up to maturation, cortex stops at elongation (5/5; 4DAG).
N70589 Name: JYB571.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS11(AT2G34710)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, stele, endodermis cortex in meristematic zone stele up to maturation, cortex stops at elongation (5/5; 4DAG).
N70590 Name: JYB572.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS11(AT2G34710)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, stele, endo cortex in meristematic zone but inconsistent in endodermis/cortex, not in cortex (2/4 ; 4DAG).
N70591 Name: JYB575.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS11(AT2G34710)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, stele, endodermis cortex in meristematic zone but inconsistent in endodermis/cortex, not in cortex (2/4; 4 and 5DAG).
N70592 Name: JYB578.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS22(AT4G28140)::cS22:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: fuzzy green signal in stele of the elongation/maturation zone (3/5; 6DAG).
N70593 Name: JYB578.2 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS22(AT4G28140)::cS22:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: fuzzy green signal in stele of the elongation/maturation zone (2/4; 6DAG).
N70594 Name: JYB579.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS22(AT4G28140)::cS22:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: fuzzy green signal in stele of the elongation/maturation zone (3/5; 6DAG), rarely on epidermis cytoplasm.
N70595 Name: JYB582.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS20(AT1G71930)::cS20:GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the xylem strand making secondary wall from late elongation zone, expression shifts from protoxylem to metaxylem (5/6; 7DAG).
N70596 Name: JYB583.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS20(AT1G71930)::cS20:GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in the xylem strand making secondary wall from late elongation zone, expression shifts from protoxylem to metaxylem (4/6; 7DAG).
N70597 Name: JYB584.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS20(AT1G71930)::cS20:GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in the xylem strand making secondary wall from late elongation zone, expression shifts from protoxylem to metaxylem (3/6; 7DAG).
N70598 Name: JYB585.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS20(AT1G71930)::cS20:GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in the xylem strand making secondary wall from late elongation zone, expression shifts from protoxylem to metaxylem (6/6; 7DAG).
N70599 Name: JYB586.1 Price: £11.00
Donor
  • Duke University Philip Benfey
  • Cornell University Ji-Young Lee
Stock type: individual line
Material type: seed


Description
Transgenic GFP-fusion expression line harboring the pS20(AT1G71930)::cS20:GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in the xylem strand making secondary wall from late elongation zone, expression shifts from protoxylem to metaxylem (4/6; 7DAG).