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The European Arabidopsis Stock Centre

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Search result for '156weedsworld '. Viewing records 1 to 50 of 216 hits.



N2106105 Name: SAND S18 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106106 Name: SAND S29 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106107 Name: SAND SUC2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106108 Name: SAND WOL Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106109 Name: SAND SHR Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106110 Name: SAND ATHB8 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106111 Name: SAND IAA19 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106112 Name: SAND DR5 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106113 Name: SAND PIN1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106114 Name: SAND PIN4 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106115 Name: SAND PIN7 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106116 Name: SAND PIN2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106117 Name: SAND WER Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106118 Name: SAND EXP7 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106119 Name: SAND PRP3 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106120 Name: SAND IRT1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106121 Name: SAND GL2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106122 Name: SAND CO2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106123 Name: SAND PEP Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106124 Name: SAND SCR Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106125 Name: SAND E47 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106126 Name: SAND UPB1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106127 Name: SAND WOX5 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106128 Name: SAND Q12 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106129 Name: SAND FEZ Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106130 Name: SAND UBQ10 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106131 Name: SAND 2x35S Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106132 Name: SAND RCH1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm
N2106133 Name: SAND KN Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106134 Name: RED TIDE S17 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106135 Name: RED TIDE S18 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106136 Name: RED TIDE S29 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106137 Name: RED TIDE S32 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106138 Name: RED TIDE SUC2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106139 Name: RED TIDE WOL Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106140 Name: RED TIDE SHR Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106141 Name: RED TIDE ATHB8 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106142 Name: RED TIDE IAA19 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106143 Name: RED TIDE DR5 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106144 Name: RED TIDE PIN1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106145 Name: RED TIDE PIN4 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106146 Name: RED TIDE WER Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106147 Name: RED TIDE EXP7 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106148 Name: RED TIDE PRP3 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106149 Name: RED TIDE IRT1 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106150 Name: RED TIDE GL2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106151 Name: RED TIDE CO2 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106152 Name: RED TIDE PEP Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106153 Name: RED TIDE SCR Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
N2106154 Name: RED TIDE E47 Price: £6.00
Donor
  • Ecole Normale Supérieure de Lyon Yvon Jaillais
  • Centre National de la Recherche Scientifique (CNRS) Gregory Vert
Locus Stock type: individual line
Material type: seed


Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus