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The European Arabidopsis Stock Centre

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Search result for 'SALK_047896 '. Viewing records 1 to 2 of 2 hits.



N547896 Name: SALK_047896 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Locus Stock type: individual line
Material type: seed


Description
Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_047896.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G27490.1 and AT1G27500.1=UTR3 (at chr 1 pos 9548620 (W/9548562-9548620) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 2, R 10, C 3 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO395271.

SALKseq_047896.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G42220.1 (at chr 3 pos 14383848 (W/14383770-14383848) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 2, R 10, C 3 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398612.

Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g49750 F14J22.4 Exon
N859846 Name: salk_047896 homozygous line Price: £11.00
Donor
  • Universitat Tubingen Wolf Frommer
  • Universitat Tubingen John M. Ward
Locus Stock type: individual line
Material type: seed


Description
confirmed homozygous line isolated from original SALK line salk_047896