Search Results
Search result for 'SALK_069823 '. Viewing records 1 to 3 of 3 hits.
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N569823 | Name: SALK_069823 | Price: £11.00 |
Donor
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Locus |
Stock type: individual line |
Material type: seed |
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Description Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_069823.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G01695.1 and AT1G01700.1 (at chr 1 pos 257862 (W/257785-257862) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359633. SALKseq_069823.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11402.1 (at chr 3 pos 3575497 (C/3575497-3575536) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 39-64 bps onto the T-DNA. Pools: P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO362988. SALKseq_069823.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G29103.1 (at chr 4 pos 14346091 (W/14346014-14346091) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO366328. SALKseq_069823.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G37000.1 (at chr 4 pos 17442563 (C/17442563-17442604) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369674. SALKseq_069823.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G58660.1 (at chr 5 pos 23703978 (W/23703901-23703978) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373034. |
Phenotype Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 |
N923641 | Name: SALK_069823.2 | Price: £11.00 |
Donor
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Locus |
Stock type: individual line |
Material type: seed |
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Description confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert. |
N923642 | Name: SALK_069823.3 | Price: £11.00 |
Donor
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Stock type: individual line |
Material type: seed |
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Description confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert. |