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Search result for 'SALK_092176 '. Viewing records 1 to 2 of 2 hits.
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N592176 | Name: SALK_092176 | Price: £11.00 |
Donor
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Locus |
Stock type: individual line |
Material type: seed |
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Description Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. December 2002 TAIR gene annotation - At2g40770 T7D17.5 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_092176.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G40770.1 (at chr 2 pos 17016930 (C/17016930-17016976) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 43-66 bps onto the T-DNA. Pools P 9, R 7, C 4 N 2. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435941. SALKseq_092176.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G09060.1 (at chr 1 pos 2922549 (C/2922549-2922611) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools: P 9, R 7, C 4 N 2. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426005. SALKseq_092176.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G03980.1=UTR3 and AT2G03990.1=P355 (at chr 2 pos 1264543 (W/1264479-1264543) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 9, R 7, C 4 N 2. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429318. SALKseq_092176.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G40770.1 (at chr 2 pos 17014180 (W/17014125-17014180) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 9, R 7, C 4 N 2. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432633. |
N923956 | Name: SALK_092176.1 | Price: £11.00 |
Donor
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Locus |
Stock type: individual line |
Material type: seed |
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Description confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert. |