Search result for 'SALK_112098 '.
Viewing records 1 to 2 of 2 hits.
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N612098
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Name: SALK_112098 |
Price:
£11.00
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Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Locus
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Stock type: individual line
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Material type: seed
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Description
Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publication
s utilizing this material.
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N923998
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Name: SALK_112098.2 |
Price:
£11.00
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Donor
- The Salk Institute for Biological Studies Joseph R. Ecker
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Locus
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Stock type: individual line
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Material type: seed
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Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
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