Search result for '16562 '.
Viewing records 1 to 5 of 5 hits.
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NW20
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Name: Landsberg erecta (Ler-0) |
Price:
£11.00
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Donor
- Wageningen University Maarten Koornneef
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Locus
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Photo
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Stock type: individual line
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Material type: seed
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Description
Line defined as erecta by Will Feenstra. Background line for all mutants produced by Feenstra, Van der Veen and Koornneef at Wageningen University
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Phenotype
blunt fruits, short petioles, usually upright stems, compact inflorescence; Ler-0, ecotype derived from Landsberg (La-0), but genetically distinct; carries erecta gene; blunt siliques, upright stems, bunched flowers; common background line. round leaves, short petioles, short pedicels, flowers are clustered at the top of the inflorescence, short and wide siliques with blunt tip, short and upright stems, compact inflorescence, carries erecta gene; height = 20-25 cm.
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N62
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Name: GA deficient |
Price:
£11.00
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Donor
- Wageningen University Maarten Koornneef
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Locus
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Stock type: individual line
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Material type: seed
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Description
Hormone
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Phenotype
Gibberellin responsive dwarf.
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N16549
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Name: 35S::GA5 in ga5-1 background |
Price:
£11.00
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Donor
- Michigan State University John Ohlrogge
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Stock type: individual line
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Material type: seed
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Description
The full length GA5 cDNA was cloned into pGEM-T Easy vector (Promega, Madison, WI) and subcloned as a XbaI-SacI fragment into pBI121 binary vector to replace the GUS gene, producing pBI121-GA5 (pBI-GA5). The construct was introduced into Agrobacterium tumefaciens strain C58C1 to transform GA4-sprayed ga5-1 Arabidopsis plants. Starting at 4-weeks and continuing weekly, ga5 plants that were to be transformed were sprayed with an aqueous solution of 10-4 M solution of GA4, containing 5 percent (v/v) ethanol. This step was necessary to overcome dwarfing and ensure fertility. Surface-sterilized T1 seeds of transformed plants were selected on MS medium supplemented with 50 microgram/ml kanamycin. Resistant seedlings were transferred to soil for continued growth and T2 seeds from several individual kanamycin-resistant plants were pooled. Successive generations (T3, T4) were harvested from plants selected on kanamycin.
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Phenotype
Dwarf phenotype of ga5-1 plants was rescued by the introduction of a wild-type copy of the gene in the 35S::GA5/ga5-1 transgenic plants.
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Donor
- California State University Judy Brusslan
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Stock type: individual line
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Material type: plasmid dna
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Description
Cloning vector. Empty plant transformation vector.
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Donor
- Michigan State University John Ohlrogge
- Algoma University Maria Isabel Molina
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Stock type: individual line
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Material type: bacterial clone
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Description
The pBI121 vector was modified by replacing the GUS reporter with the GA5 cDNA. This construct can be used for plant transformation to complement the ga5-1 mutation.
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