NASC

N2106105

Name: SAND S18

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106106

Name: SAND S29

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106107

Name: SAND SUC2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106108

Name: SAND WOL

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106109

Name: SAND SHR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106110

Name: SAND ATHB8

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106111

Name: SAND IAA19

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106112

Name: SAND DR5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus synthetic

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106113

Name: SAND PIN1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106114

Name: SAND PIN4

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106115

Name: SAND PIN7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106116

Name: SAND PIN2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106117

Name: SAND WER

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106118

Name: SAND EXP7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106119

Name: SAND PRP3

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106120

Name: SAND IRT1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106121

Name: SAND GL2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106122

Name: SAND CO2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106123

Name: SAND PEP

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106124

Name: SAND SCR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106125

Name: SAND E47

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106126

Name: SAND UPB1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106127

Name: SAND WOX5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106128

Name: SAND Q12

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106129

Name: SAND FEZ

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106130

Name: SAND UBQ10

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106131

Name: SAND 2x35S

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus Cauliflower mosaic virus promoter

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106132

Name: SAND RCH1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106133

Name: SAND KN

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus