Search result for '2110797 '.
Viewing records 1 to 12 of 12 hits.
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N2110785
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Name: MAP-mCH-dOCRL |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction.
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N2110786
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Name: MAP-mCH-dOCRLdead |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a control line for the iDePP system. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. The MAP-mCH-dOCRLdead is a catalyticaly inactive control.
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a control line for the iDePP system. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. MAP-3xmCHERRY is a generic control to make sure that the observed phenotype are not caused by the induction of the transgene upon dex treatment.
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N2110788
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Name: MAP-mCH-dOCRL x mCit-2xPH(PLC) |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross that allows evaluating the dynamics and cell type specificty of PI(4,5)P2 removal when using iDePP. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction.This line is the result from a cross with the high affitnity PI(4,5)P2 sensor P24Y (NASC ID: N2105613), 2xPH(PLC), and it allows to follow PI(4,5)P2 remooval using live imaging upon induction of iDePP.
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N2110789
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Name: MAP-mCH-dOCRLdead x mCit-2xPH(PLC) |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that removal of PI(4,5)P2 from the plasma membrane by iDePP is due to dOCRL catalytic activity. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. The MAP-mCH-dOCRLdead is a control (catalyticaly inactive). This line is the result from a cross with the high affitnity PI(4,5)P2 sensor P24Y (NASC ID: N2105613), 2xPH(PLC).
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N2110790
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Name: MAP-3xmCH x mCit-2xPH(PLC) |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that removal of PI(4,5)P2 from the plasma membrane by iDePP is not a side effect from dexamathasone treatment. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. MAP-3xmCHERRY is a control to make sure that the observed phenotype are not due induction of a transgene upon dex treatment. This line is the result from a cross with the high affitnity PI(4,5)P2 sensor P24Y (NASC ID: N2105613), 2xPH(PLC).
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N2110791
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Name: MAP-mCH-dOCRL x mCit-TUBBY |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
|
Phenotype
This is a cross, which allows evaluating the dynamics and cell type specificty of PI(4,5)P2 removal when using iDePP. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction.This line is the result from a cross with the PI(4,5)P2 sensor P25Y (NASC ID: N2105610), TUBBY-C, and it allows to follow PI(4,5)P2 remooval using live imaging upon induction of iDePP.
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N2110792
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Name: MAP-mCH-dOCRLdead x mCit-TUBBY |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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|
Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that removal of PI(4,5)P2 from the plasma membrane by iDePP is due to dOCRL catalytic activity. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. The MAP-mCH-dOCRLdead is a control (catalyticaly inactive). This line is the result from a cross with the PI(4,5)P2 sensor P25Y (NASC ID: N2105610), TUBBY-C.
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N2110793
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Name: MAP-3xmCH x mCit-TUBBY |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that removal of PI(4,5)P2 from the plasma membrane by iDePP is not a side effect from dexamathasone treatment. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. MAP-3xmCHERRY is a control to make sure that the observed phenotype are not due induction of a transgene upon dex treatment. This line is the result from a cross with the PI(4,5)P2 sensor P25Y (NASC ID: N2105610), TUBBY-C.
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N2110794
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Name: MAP-mCH-dOCRL x mCit-P4M |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that iDePP is highly specific for PI(4,5)P2 and has no impact on PI4P at the plamsma membrane. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction.This line is a control. It is the result from a cross with the PI4P sensor mCit-P4M (NASC ID: N2107346), and it allows to check that iDePP has no effect on PI4P accumulation at the plasma membrane.
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N2110795
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Name: MAP-mCH-dOCRLdead x mCit-P4M |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a control corss for the iDePP system showing that induction of inactive (dead) OCRL has no impact on PI4P. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. The MAP-mCH-dOCRLdead is a control (catalyticaly inactive). It is the result from a cross with the PI4P sensor mCit-P4M (NASC ID: N2107346), and it allows to check that iDePP has no effect on PI4P accumulation at the plasma membrane.
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N2110796
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Name: MAP-3xmCH x mCit-P4M |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
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|
Stock type: individual line
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Material type: seed
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Description
Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. This line is part of the set N2110797.
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Phenotype
This is a cross, which allows evaluating that removal of PI(4,5)P2 from the plasma membrane by iDePP is not a side effect from dexamathasone treatment and is specific to this lipid and not PI4P. iDePP is a system for the inducible depletion of PI(4,5)P2 in Arabidopsis. It consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylatoion and Palmitoylation) and a mCHERRY for visualisation. iDePP is dex (dexamethasone) inducible and impacts the steady state of PI(4,5)P2 starting few hours after induction. MAP-3xmCHERRY is a control to make sure that the observed phenotype are not due induction of a transgene upon dex treatment. This line is the result from a cross with the PI4P sensor mCit-P4M (NASC ID: N2107346), and it allows to check that iDePP has no effect on PI4P accumulation at the plasma membrane.
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