NASC Stock Detail Page
NASC ID: N2107349
Name: INT
ABRC stock number: CS2107349
Description: NTF and BirA independent lines were generated by transformation using Agrobaterium with pPHE::NTF and pPHE::BirA constructs respectively. Transgenic lines were selected in vitro on ppt (basta). Selected lines with appropriate NTF and BirA levels were cross-pollinated and homozygous lines selected (using the GFP fluorescence signal of the NTF construct and specific PCR-primers for the BirA transgene). The pPHE::NTF; pPHE::BirA doble transgenic line has been named as INT.
Donation Date: 2016-07-20
| Donated by: | Swedish University of Agricultural Sciences (SLU) Jordi Moreno-Romero Swedish University of Agricultural Sciences (SLU) Claudia Kohler |
Donor Number: pPHE::NTF; pPHE::BirA
Stock type: individual line
Material type: seed
Comment: Donor name: Claudia Köhler, Jordi Moreno-Romero
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Mutagen: ema
Background: Col-0
Segregation status: homozygous
Growth requirement: none
Phenotype
Endosperm-specific promoter expressing NTF and BirA allowing nuclei purification with the INTACT method.
References
Moreno-Romero, J. et al. 2016. Parental epigenetic asymmetry of PRC2-mediated histone modifications in the Arabidopsis endosperm. EMBO Journal 35(12):1298-311. Pubmed ID: 27113256