NASC Stock Detail Page
NASC ID: N2107350
Name: dde2; INT
ABRC stock number: CS2107350
Description: dde2 line (SALK_017756) was crossed with INT line and triple homozygous (NTF transgene, BirA transgene and dde2 mutant) were selected.
Donation Date: 2016-07-20
| Donated by: | Swedish University of Agricultural Sciences (SLU) Jordi Moreno-Romero Swedish University of Agricultural Sciences (SLU) Claudia Kohler |
Donor Number: dde2; pPHE::NTF; pPHE::BirA
Stock type: individual line
Material type: seed
Comment: Donor name: Claudia Köhler, Jordi Moreno-Romero
| Status | Price (£) |
|---|---|
| Currently unavailable | £11.00 |
Germplasm Info
Mutagen: ema
Background: dde2 (SALK_017756) in Col-0
Segregation status: homozygous
Growth requirement: The influorescences has to be sprayed with MeJA for self-fertilization
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
The dde2-2 mutant is defective in the anther dehiscence process and thus flowers are not self pollinated and seeds are not produced. The INT transgenic line has an endosperm-specific promoter expressing NTF and BirA allowing nuclei purification with the INTACT method.
References
Moreno-Romero, J. et al. 2016. Parental epigenetic asymmetry of PRC2-mediated histone modifications in the Arabidopsis endosperm. EMBO Journal 35(12):1298-311. Pubmed ID: 27113256
Przybyla, D. et al. 2008. Enzymatic, but not non-enzymatic, 1O2-mediated peroxidation of polyunsaturated fatty acids forms part of the EXECUTER1-dependent stress response program in the flu mutant of Arabidopsis thaliana. The Plant Journal 54(2):236-48. Pubmed ID: 18182022.