NASC

NASC ID: N2109736

Name: mEos-ROP6(CA)

ABRC stock number: CS2109736

Description: Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28 Celsius, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3.

Donation Date: 2019-04-01

Donated by:

Ecole Normale Supérieure de Lyon Yvon Jaillais

Donor Number: 2x35S::mEos-ROP6(CA)

Stock type: individual line

Material type: seed

Comment: Gene: Rho of Plant 6 (ROP6)

Status	Price (£)
Available	£11.00

Germplasm Info

Seed type: Transgenic

Background: Col0

Segregation status: homozygous

Growth requirement: none

Transformation method: Agrobacterium-mediated transformation

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		At4g35020	At4g35020

Phenotype

Overexpression of a constitutive active (CA) allele of ROP6. ROP6 is fused with mEos for PALM analysis. (internal line code 1139-1)

References

Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, Maria del Mar Marquès-Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nollmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais; 2019-04-05; Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine.; Science; PubMed ID: 30948546