NASC Stock Detail Page
NASC ID: N2109741
Name: mCITRINE-ROP6(7Q)
ABRC stock number: CS2109741
Description: Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28 Celsius, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3.
Donation Date: 2019-04-01
| Donated by: | Ecole Normale Supérieure de Lyon Yvon Jaillais |
Donor Number: ROP6prom::mCITRINE-ROP6(7Q)
Stock type: individual line
Material type: seed
Comment: Gene: Rho of Plant 6 (ROP6)
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: rop6-2
Segregation status: homozygous
Growth requirement: none
Transformation method: Agrobacterium-mediated transformation
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Non complemented rop6-2 line. Construct span the entire ROP6 genomic fragment containing 5' and 3' intergenic regions, with mCITRINE fused just before ROP6 START codon. ROP6 C-terminal tail is mutated into 7 Gln (internal line code 1154-4)
References
Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, Maria del Mar Marquès-Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nollmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais; 2019-04-05; Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine.; Science; PubMed ID: 30948546