NASC Stock Detail Page
NASC ID: N2109747
Name: ami1 PSS1
ABRC stock number: CS2109747
Description: Each construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28 Celsius, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta) or Hygromycine. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3.
Donation Date: 2019-04-01
Donated by: | Ecole Normale Supérieure de Lyon Yvon Jaillais |
Donor Number: UBQ10prom::amiPSS1 #1
Stock type: individual line
Material type: seed
Comment: Gene: PHOSPHATIDYLSERINE SYNTHASE 1 (PSS1)
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Col0
Segregation status: homozygous
Growth requirement: none
Transformation method: Agrobacterium-mediated transformation
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
artificial microRNA against PSS1. Line is amiPSS1 line 1 (different microRNA than line 2). (internal line code 1175-15)
References
Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, Maria del Mar Marquès-Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nollmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais; 2019-04-05; Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine.; Science; PubMed ID: 30948546