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NASC Stock Detail Page

NASC ID: N2110920

Name: asl9

ABRC stock number: CS2110920

Description: asl9-1 has a premature stop codon at the 19th codon via an insertion of a single nucleotide “A”. This was generated using CRISPR-CAS9 system. pJY-35SpCas9-RG1 binary construct was used to target LBD3/ASL9 via agrobacterium-mediated transformation, in which 35S promoter and AtU6 promoter drive Cas9 and sgRNA, respectively. Three 20-mer targeting regions were selected for CRISPR guide sequences, based on the prediction results from CRISPR RGEN Tools (www.rgenome.net): RG1 (5’-AGACAAAAGGGTCACAGACA-3’); RG2 (5’-TCGCCGGAGAAGTTTACAGC-3’); and RG3 (5’-GGCTCTTTGGCCGGAAAATA -3’). Eight hygromycin-resistant transgenic T1 plants harbouring the CRISPR cassette either with sgRNA-RG1, RG2, or RG3 were grown for T2 germline transmission of potentially edited alleles. Sanger sequencing analyses on targeted regions from T2 progenies revealed that targeted RG1successfully generated homozygous asl9-1 allele. After confirming the mutation, T-DNA containing CRISPR-CAS9 was removed by outcrossing to the Col-0 wild type..

Donation Date: 2021-11-02

Donated by:  Seoul National University Ji-Young Lee
Seoul National University Jung-Hun Lee

Donor Number: asl9

Stock type: individual line

Material type: seed

Status Price (£)
Available £10.00

Germplasm Info

Seed type: Transgenic

Background: Col

Segregation status: homozygous

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • LBD3/ASL9

Phenotype

Slight reduction in stem and root diameters due to reduced cambial activities; flowering is a bit faster than the wild type when grown at 22 degree with 16hr/8hr of day/night cycle

References

Jing Zhang , Gugan Eswaran , Juan Alonso-Serra, Melis Kucukoglu, Jiale Xiang, Weibing Yang, Annakaisa Elo, Kaisa Nieminen, Teddy Damén, Je-Gun Joung, Jae-Young Yun, Jung-Hun Lee, Laura Ragni, Pierre Barbier de Reuille, Sebastian E Ahnert, Ji-Young Lee, Ari Pekka Mähönen, Ykä Helariutta. ; 2019-10-05; Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots; Nature Plants; PubMed ID: 31595065