NASC

NASC ID: N529648

Name: SALK_029648

ABRC stock number: SALK_029648

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_029648.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G51300.1 (at chr 1 pos 19015132 (C/19015132-19015195) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 3, R 2, C 9 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO364241.

SALKseq_029648.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G11870.1 and AT3G11880.1=P355 (at chr 3 pos 3750312 (W/3750234-3750312) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 3, R 2, C 9 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO367588.

SALKseq_029648.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G23810.1=UTR3 and AT5G23820.1=P355 (at chr 5 pos 8030821 (C/8030821-8030870) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 3, R 2, C 9 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO370938.

SALKseq_029648.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G24850.1 (at chr 5 pos 8538153 (C/8538153-8538231) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 3, R 2, C 9 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374285.

Donation Date: 2001-01-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_029648

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_029648C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G11870 AT3G11880 AT5G23810 AT5G23820 AT5G24850 At1g51300

EMBL accession:

BH753792

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g51300 F11M15.15 Intron : gene annotation - At1g51300

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.