NASC Stock Detail Page
NASC ID: N531535
Name: SALK_031535
ABRC stock number: SALK_031535
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_031535.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G32450.1 (at chr 2 pos 13778458 (W/13778409-13778458) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 8, R 3, C 2 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO384441. SALKseq_031535.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G66150.1 (at chr 1 pos 24631685 (W/24631628-24631685) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 8, R 3, C 2 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381091. SALKseq_031535.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G05230.1=P355 and AT2G05240.1 (at chr 2 pos 1907370 (W/1907293-1907370) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 8, R 3, C 2 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO387788. SALKseq_031535.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G54510.1 (at chr 3 pos 20181524 (W/20181471-20181524) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 8, R 3, C 2 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391129.
Donation Date: 2001-01-12
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_031535
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662020 (SALK_031535C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g32450
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.