NASC

NASC ID: N533390

Name: SALK_033390

ABRC stock number: SALK_033390

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_033390.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G58620.1 (at chr 3 pos 21681829 (W/21681771-21681829) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 1, R 9, C 6 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374481.

SALKseq_033390.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G71710.2=P355 and AT1G71720.1=P355 (at chr 1 pos 26981949 (W/26981871-26981949) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 1, R 9, C 6 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364437.

SALKseq_033390.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G27395.1 (at chr 5 pos 9671843 (W/9671779-9671843) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 1, R 9, C 6 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO377830.

SALKseq_033390.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G76000 and AT1G76010.1=UTR3 (at chr 1 pos 28527880 (W/28527816-28527880) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 1, R 9, C 6 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO367776.

SALKseq_033390.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28060.1=P355 and AT2G28070.1 (at chr 2 pos 11954626 (W/11954563-11954626) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 1, R 9, C 6 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371132.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_033390

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662049 (SALK_033390C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G71710 AT1G71720 AT1G76000 AT1G76010 AT2G28060 AT2G28070 AT5G27395 At3g58620

EMBL accession:

ED580365

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g58620

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.