NASC

NASC ID: N534354

Name: SALK_034354

ABRC stock number: SALK_034354

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_034354.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G32060.1 (at chr 2 pos 13639231 (W/13639173-13639231) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 3, R 5, C 5 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374529.

SALKseq_034354.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G05360.1 (at chr 1 pos 1567123 (W/1567070-1567123) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 3, R 5, C 5 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO367822.

SALKseq_034354.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G43700.1 (at chr 3 pos 15603451 (C/15603451-15603495) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 3, R 5, C 5 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO377877.

SALKseq_034354.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G10522.2 (at chr 1 pos 3469587 (C/3469587-3469652) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 3, R 5, C 5 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371178.

SALKseq_034354.201 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G60897.1 and AT3G60900.1=UTR3 (at chr 3 pos 22499299 (C/22499299-22499353) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 3, R 5, C 5 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381215.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_034354

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665733 (SALK_034354C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G05360 AT1G10522 AT3G43700 AT3G60897 AT3G60900 At2g32060

EMBL accession:

ED581050

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g32060

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.