NASC

NASC ID: N539004

Name: SALK_039004

ABRC stock number: SALK_039004

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039004.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51630.1 (at chr 3 pos 19149540 (W/19149477-19149540) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 10, R 4, C 10 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO364717.

SALKseq_039004.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G06165.1 (around chr 2 pos 2410627 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414879.

SALKseq_039004.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G07780.1 (around chr 2 pos 3593336 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414880.

SALKseq_039004.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G43526.1 (at chr 3 pos 15422809 (C/15422809-15422887) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 10, R 4, C 10 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361356.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039004

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654152 (SALK_039004C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G06165 AT2G07780 AT3G43526 At3g51630

EMBL accession:

ED583930

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g51630

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.