NASC Stock Detail Page
NASC ID: N540045
Name: SALK_040045
ABRC stock number: SALK_040045
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_040045.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G34131.1 (at chr 4 pos 16344972 (W/16344914-16344972) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 13, R 13, C 15 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401582. SALKseq_040045.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G13800.1=UTR3 and AT2G13810.1=P355 (around chr 2 pos 5765630 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414917. SALKseq_040045.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34220.1=P355 and AT4G34230.2=UTR3 (at chr 4 pos 16385059 (W/16384994-16385059) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 13, R 13, C 15 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO404909. SALKseq_040045.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G61210.1=P355 and AT3G61220.2=P355 (at chr 3 pos 22660270 (W/22660192-22660270) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 13, R 13, C 15 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394905. SALKseq_040045.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G61210.1=P355 and AT3G61220.2=P355 (at chr 3 pos 22660289 (C/22660289-22660326) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 35-73 bps onto the T-DNA. Pools P 13, R 13, C 15 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398240.
Donation Date: 2002-01-01
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_040045
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_040045C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g34131
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.