NASC Stock Detail Page
NASC ID: N540299
Name: SALK_040299
ABRC stock number: SALK_040299
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_040299.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G50300.1=P355 and AT1G50310.1 (at chr 1 pos 18632827 (C/18632827-18632905) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 12, R 20, C 19 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO361432. SALKseq_040299.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G16950.1=UTR3 and AT1G16960.1=P355 (around chr 1 pos 5799307 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414933. SALKseq_040299.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G03470.1 (at chr 1 pos 866069 (W/866002-866069) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 12, R 20, C 19 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO368128. SALKseq_040299.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G44605 and AT3G44610.1=UTR3 (at chr 3 pos 16185978 (C/16185978-16186056) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 12, R 20, C 19 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371489.
Donation Date: 2002-01-01
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_040299
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653157 (SALK_040299C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g03470
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.