NASC Stock Detail Page
NASC ID: N543121
Name: SALK_043121
ABRC stock number: SALK_043121
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_043121.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G19360.1 (at chr 3 pos 6708059 (W/6708006-6708059) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 15, R 11, C 13 N 11. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO344808. SALKseq_043121.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G36080.1 (at chr 4 pos 17064394 (C/17064394-17064472) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 11, C 13 N 11. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348161. SALKseq_043121.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G13222.1 (at chr 3 pos 4251308 (C/4251308-4251368) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 15, R 11, C 13 N 11. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411706. SALKseq_043121.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G16000.1 (at chr 3 pos 5430948 (W/5430887-5430948) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 15, R 11, C 13 N 11. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO341453.
Donation Date: 2002-01-01
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_043121
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655471 (SALK_043121C).
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.