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NASC ID: N543937

Name: SALK_043937

ABRC stock number: SALK_043937

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_043937.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G46970.1 (at chr 2 pos 19296540 (W/19296486-19296540) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 13, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO381670.

SALKseq_043937.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G04510.1 (at chr 3 pos 1216144 (W/1216107-1216144) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools: P 13, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385020.

SALKseq_043937.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G02000.1=P355 and AT3G02010.1 (at chr 3 pos 335279 (W/335248-335279) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 38-59 bps onto the T-DNA. Pools P 13, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388368.

SALKseq_043937.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G20000.1 (at chr 5 pos 6756747 (C/6756747-6756825) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 13, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391700.

Donation Date: 2002-01-01

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_043937

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653169 (SALK_043937C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g46970

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.