NASC Stock Detail Page
NASC ID: N545625
Name: SALK_045625
ABRC stock number: SALK_045625
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_045625.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30220.1=P355 and AT1G30230.1=P355 (at chr 1 pos 10637012 (C/10637012-10637063) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 14, R 16, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO344949. SALKseq_045625.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G70450.1 and AT1G70460.1=P355 (at chr 1 pos 26554752 (W/26554686-26554752) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 14, R 16, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348300. SALKseq_045625.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22410.1=UTR3 and AT2G22420.1=P355 (at chr 2 pos 9512329 (W/9512251-9512329) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 14, R 16, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351655. SALKseq_045625.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G27040.1 (at chr 3 pos 9975773 (W/9975723-9975773) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 57-77 bps onto the T-DNA. Pools P 14, R 16, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354984.
Donation Date: 2002-01-01
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_045625
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_045625C.
Status | Price (£) |
---|---|
Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.