NASC Stock Detail Page
NASC ID: N546366
Name: SALK_046366
ABRC stock number: SALK_046366
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: T30C3.8 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g25510. Associated Polymorphisms: SALKseq_046366.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G25515.1 (at chr 4 pos 13028520 (C/13028520-13028582) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 15, R 11, C 11 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO355026. SALKseq_046366.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G24920.1 (at chr 4 pos 12820738 (W/12820684-12820738) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 15, R 11, C 11 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351694.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_046366
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N658218 (SALK_046366C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.