NASC Stock Detail Page
NASC ID: N546633
Name: SALK_046633
ABRC stock number: SALK_046633
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_046633.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G06400.1 (at chr 1 pos 1951537 (W/1951470-1951537) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 18, R 12, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO371773. SALKseq_046633.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G17650.1 (at chr 1 pos 6070052 (W/6070021-6070052) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 28-50 bps onto the T-DNA. Pools P 18, R 12, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO375127. SALKseq_046633.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G51550.1 (at chr 3 pos 19120800 (C/19120800-19120861) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 18, R 12, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378475. SALKseq_046633.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51895.1 (at chr 3 pos 19255703 (W/19255674-19255703) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 18, R 12, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381821.
Donation Date: 2002-01-02
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_046633
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N656456 (SALK_046633C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g06400 T2D23.10 Intron
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.