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NASC Stock Detail Page

NASC ID: N547427

Name: SALK_047427

ABRC stock number: SALK_047427

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_047427.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G41080.1 (at chr 5 pos 16441592 (C/16441592-16441628) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 19, R 20, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO365117.

SALKseq_047427.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G46140.1=UTR3 and AT2G46150.1=P355 (at chr 2 pos 18960999 (C/18960999-18961039) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 19, R 20, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361763.

Donation Date: 2002-01-02

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_047427

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653183 (SALK_047427C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At5g41080 MEE6.15 L300-5'

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.