NASC Stock Detail Page
NASC ID: N547512
Name: SALK_047512
ABRC stock number: SALK_047512
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_047512.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G30880.1 (at chr 1 pos 10993783 (W/10993740-10993783) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 16, R 19, C 17 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO381865. SALKseq_047512.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G18850.1=P355 and AT1G18860.1=P355 (at chr 1 pos 6508645 (C/6508645-6508693) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 16, R 19, C 17 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385217. SALKseq_047512.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G03630.1 (at chr 1 pos 907607 (W/907556-907607) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 16, R 19, C 17 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388563. SALKseq_047512.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G13490.1 (at chr 5 pos 4335413 (W/4335363-4335413) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 49-79 bps onto the T-DNA. Pools P 16, R 19, C 17 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391897.
Donation Date: 2002-01-02
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_047512
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655497 (SALK_047512C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g30880 F17F8.25 Exon
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.