NASC Stock Detail Page
NASC ID: N549062
Name: SALK_049062
ABRC stock number: SALK_049062
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: T16L1.30 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g33540. Associated Polymorphisms: SALKseq_049062.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G33530.1 (at chr 4 pos 16130631 (W/16130570-16130631) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO351833. SALKseq_049062.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G40230.1 (at chr 2 pos 16803109 (W/16803066-16803109) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 20, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345132. SALKseq_049062.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G35460.1=UTR3 and AT4G35470.1=P355 (at chr 4 pos 16845398 (W/16845339-16845398) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO355167. SALKseq_049062.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G22310.1 (at chr 4 pos 11791329 (W/11791274-11791329) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 20, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348476.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_049062
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_049062C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.