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The European Arabidopsis Stock Centre

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NASC ID: N551473

Name: SALK_051473

ABRC stock number: SALK_051473

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_051473.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G74520.1 (at chr 1 pos 28009266 (W/28009216-28009266) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 41-77 bps onto the T-DNA. Pools P 23, R 28, C 25 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378691.

SALKseq_051473.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G20260.1 (at chr 1 pos 7020203 (W/7020147-7020203) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 47-76 bps onto the T-DNA. Pools P 23, R 28, C 25 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO382035.

SALKseq_051473.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G02830 and AT2G02835.1 (at chr 2 pos 818310 (C/818310-818388) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 28, C 25 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO385388.

SALKseq_051473.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G28080.1 (at chr 4 pos 13951685 (C/13951685-13951737) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 23, R 28, C 25 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388735.

SALKseq_051473.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G29310.1=P355 and AT4G29330.1=P355 (at chr 4 pos 14441060 (W/14440983-14441060) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 28, C 25 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO392068.

Donation Date: 2002-01-02

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_051473

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_051473C.

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g74520 F1M20.20 R300-5'

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.