NASC

NASC ID: N553127

Name: SALK_053127

ABRC stock number: SALK_053127

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_053127.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G01810.1 (at chr 3 pos 288889 (W/288818-288889) on the TAIR10). The sequences' 1-72 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 24, R 23, C 28 N 24. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355331.

SALKseq_053127.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G05250.1=P355 and AT1G05260.1=P355 (at chr 1 pos 1529254 (W/1529183-1529254) on the TAIR10). The sequences' 1-72 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 24, R 23, C 28 N 24. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO348640.

SALKseq_053127.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04770 and AT2G04780.1=P355 (at chr 2 pos 1674380 (C/1674380-1674460) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 24, R 23, C 28 N 24. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO351997.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_053127

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N658241 (SALK_053127C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G05250 AT1G05260 AT2G04770 AT2G04780 At3g01810

EMBL accession:

BH847397

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.