NASC Stock Detail Page
NASC ID: N554351
Name: SALK_054351
ABRC stock number: SALK_054351
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: F21F14.130 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g61960. Associated Polymorphisms: SALKseq_054351.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G61960.1 (at chr 3 pos 22942459 (C/22942459-22942513) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 26, R 29, C 23 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362077. SALKseq_054351.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G19640.1 (at chr 2 pos 8492940 (W/8492877-8492940) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 26, R 29, C 23 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355394. SALKseq_054351.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G37110.1 (at chr 4 pos 17486287 (C/17486287-17486343) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 26, R 29, C 23 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365428. SALKseq_054351.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G36659.1 and AT3G06660.1=P355 (at chr 3 pos 2101180 (W/2101117-2101180) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 26, R 29, C 23 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358733. SALKseq_054351.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G39410.1 and AT4G39420.1=P355 (at chr 4 pos 18338704 (C/18338704-18338757) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 26, R 29, C 23 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368772.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_054351
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_054351C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.