NASC Stock Detail Page
NASC ID: N555462
Name: SALK_055462
ABRC stock number: SALK_055462
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.March 2002 TAIR gene annotation: MDN11.16 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g48080 Associated Polymorphisms: SALKseq_055462.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G48090.1 (at chr 5 pos 19483982 (W/19483927-19483982) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 30, R 21, C 27 N 29. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348764. SALKseq_055462.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G03935=P355 and AT4G03945.1=P355 (at chr 4 pos 1877496 (W/1877419-1877496) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 30, R 21, C 27 N 29. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345420. SALKseq_055462.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G22740.1 (at chr 5 pos 7555168 (W/7555099-7555168) on the TAIR10). The sequences' 1-70 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 30, R 21, C 27 N 29. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO352121.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_055462
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653217 (SALK_055462C).
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.