NASC

NASC ID: N555573

Name: SALK_055573

ABRC stock number: SALK_055573

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: MBK21.12 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g12740.

Associated Polymorphisms:

SALKseq_055573.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G12740.1 (at chr 3 pos 4051570 (W/4051495-4051570) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 30, R 21, C 24 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342070.

SALKseq_055573.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G27930.1 (at chr 1 pos 9732880 (W/9732806-9732880) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 30, R 21, C 24 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405638.

SALKseq_055573.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G47330.1=P355 and AT2G47340.1 (at chr 2 pos 19432114 (W/19432048-19432114) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 30, R 21, C 24 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO408975.

SALKseq_055573.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G42530.1 (at chr 3 pos 14636060 (C/14636060-14636154) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 30, R 21, C 24 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412325.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_055573

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_055573C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G27930 AT2G47330 AT2G47340 AT3G42530 At3g12740

EMBL accession:

BH757185

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.