NASC

NASC ID: N557152

Name: SALK_057152

ABRC stock number: SALK_057152

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_057152.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G33360.1 (at chr 2 pos 14139652 (W/14139592-14139652) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 31, R 35, C 38 N 38. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO375626.

SALKseq_057152.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51940.1 (at chr 3 pos 19273743 (W/19273691-19273743) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 31, R 35, C 38 N 38. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378973.

SALKseq_057152.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G32540.1=UTR3 and AT4G32551.2=P355 (at chr 4 pos 15705463 (W/15705404-15705463) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 31, R 35, C 38 N 38. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO382319.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_057152

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_057152C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At2g33360 AT3G51940 AT4G32540 AT4G32551

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.