NASC

NASC ID: N557365

Name: SALK_057365

ABRC stock number: SALK_057365

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: MXC17.10 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g24710.

Associated Polymorphisms:

SALKseq_057365.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G24710.1 (at chr 5 pos 8459291 (W/8459231-8459291) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 31, R 38, C 33 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375636.

SALKseq_057365.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G01520.1 and AT1G01530.1 (at chr 1 pos 192559 (W/192495-192559) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 31, R 38, C 33 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362235.

SALKseq_057365.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G71190.1 (at chr 1 pos 26834633 (W/26834555-26834633) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 31, R 38, C 33 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365583.

SALKseq_057365.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G19830.1=UTR3 and AT1G19835.1=UTR3 (at chr 1 pos 6854269 (W/6854191-6854269) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 31, R 38, C 33 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368928.

SALKseq_057365.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G30750.1=P355 and AT2G30760.1 (at chr 2 pos 13102988 (W/13102941-13102988) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 31, R 38, C 33 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372284.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_057365

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_057365C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G01520 AT1G01530 AT1G19830 AT1G19835 AT1G71190 AT2G30750 AT2G30760 At5g24710

EMBL accession:

BH790582

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.