NASC Stock Detail Page
NASC ID: N558170
Name: SALK_058170
ABRC stock number: SALK_058170
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: MMN10.18 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g60010. Associated Polymorphisms: SALKseq_058170.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G60010.1 (at chr 5 pos 24163464 (W/24163387-24163464) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 35, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365630. SALKseq_058170.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30040.1=UTR3 and AT1G30050.1=P355 (at chr 1 pos 10540703 (W/10540626-10540703) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 35, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355595. SALKseq_058170.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G17695.1 (at chr 2 pos 7685334 (W/7685257-7685334) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 35, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358937. SALKseq_058170.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16820.1 and AT4G16830.1=P355 (at chr 4 pos 9469300 (W/9469223-9469300) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 35, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362281.
Donation Date: 2002-04-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_058170
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654670 (SALK_058170C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.