NASC

NASC ID: N558449

Name: SALK_058449

ABRC stock number: SALK_058449

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T30C3.7 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g25430.

Associated Polymorphisms:

SALKseq_058449.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G25430.1 (at chr 4 pos 12999524 (C/12999524-12999584) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 32, R 40, C 38 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO348922.

SALKseq_058449.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G32090.2=UTR3 and AT2G32100.1 (at chr 2 pos 13646876 (W/13646815-13646876) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 32, R 40, C 38 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342222.

SALKseq_058449.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G48120.1 (at chr 5 pos 19513118 (W/19513059-19513118) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 32, R 40, C 38 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352278.

SALKseq_058449.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G04590.1 (at chr 3 pos 1239115 (C/1239115-1239157) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 32, R 40, C 38 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345577.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_058449

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N658257 (SALK_058449C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G32090 AT2G32100 AT3G04590 AT5G48120 At4g25430

EMBL accession:

ED590736

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.