NASC Stock Detail Page
NASC ID: N558704
Name: SALK_058704
ABRC stock number: SALK_058704
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: MJK13.12 R300-5' At3g15470 MJK13.13 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g15460. Associated Polymorphisms: SALKseq_058704.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G15460.1 (at chr 3 pos 5216419 (W/5216361-5216419) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 33, R 31, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342234. SALKseq_058704.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G55440.1=UTR3 and AT3G55450.1=P355 (at chr 3 pos 20557144 (C/20557144-20557178) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 33, R 31, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412492. SALKseq_058704.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G24590.1 (at chr 4 pos 12696502 (W/12696448-12696502) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 53-74 bps onto the T-DNA. Pools P 33, R 31, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345590.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_058704
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_058704C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.