NASC

NASC ID: N560808

Name: SALK_060808

ABRC stock number: SALK_060808

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F1N13.140 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g16000.

Associated Polymorphisms:

SALKseq_060808.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G16000.1 (at chr 5 pos 5225671 (W/5225613-5225671) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 34, R 37, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382523.

SALKseq_060808.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28400=P355 and AT3G28410.1 (at chr 3 pos 10639425 (W/10639378-10639425) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 41-70 bps onto the T-DNA. Pools P 34, R 37, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375835.

SALKseq_060808.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G42950.1 (at chr 5 pos 17225074 (C/17225074-17225151) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 34, R 37, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379181.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_060808

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_060808C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G28400 AT3G28410 AT5G42950 At5g16000

EMBL accession:

BH791665

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.