NASC Stock Detail Page
NASC ID: N562218
Name: SALK_062218
ABRC stock number: SALK_062218
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: MNA5.13 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g65400. Associated Polymorphisms: SALKseq_062218.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19730.1 and AT4G19740.1 (at chr 4 pos 10737164 (C/10737164-10737193) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 49-78 bps onto the T-DNA. Pools P 37, R 35, C 36 N 31. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO382611. SALKseq_062218.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G65400.1 (at chr 5 pos 26132916 (W/26132869-26132916) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 37, R 35, C 36 N 31. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385965. SALKseq_062218.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G30730.1 (at chr 2 pos 13094393 (C/13094393-13094455) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 37, R 35, C 36 N 31. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO379269.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_062218
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653732 (SALK_062218C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.