NASC

NASC ID: N563592

Name: SALK_063592

ABRC stock number: SALK_063592

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F18O22.30 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g14240.

Associated Polymorphisms:

SALKseq_063592.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G14240.1 (at chr 5 pos 4597657 (W/4597615-4597657) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 49-78 bps onto the T-DNA. Pools P 39, R 37, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355907.

SALKseq_063592.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G16680.1 (at chr 3 pos 5683243 (W/5683189-5683243) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 39, R 37, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345877.

SALKseq_063592.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G02930.2=UTR3 and AT3G02940.1=P355 (at chr 3 pos 658657 (C/658657-658706) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 39, R 37, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349221.

SALKseq_063592.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G58100.1 (at chr 5 pos 23507642 (W/23507596-23507642) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 39, R 37, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352578.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_063592

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_063592C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G02930 AT3G02940 AT3G16680 AT5G58100 At5g14240

EMBL accession:

ED594010

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.