NASC

NASC ID: N563797

Name: SALK_063797

ABRC stock number: SALK_063797

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T12H17.100 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g22710.

Associated Polymorphisms:

SALKseq_063797.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G22720.1 (at chr 4 pos 11936649 (W/11936599-11936649) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 48-78 bps onto the T-DNA. Pools P 38, R 38, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355918.

SALKseq_063797.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28200.1=UTR3 and AT2G28210.1 (at chr 2 pos 12026026 (C/12026026-12026086) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 38, R 38, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352591.

SALKseq_063797.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G23915 and AT4G23920.1=P355 (at chr 4 pos 12430245 (C/12430245-12430288) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 48-78 bps onto the T-DNA. Pools P 38, R 38, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359264.

SALKseq_063797.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G22020.1 (at chr 5 pos 7287894 (C/7287894-7287930) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 38, R 38, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362611.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_063797

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_063797C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G28200 AT2G28210 AT4G22720 AT4G23915 AT4G23920 AT5G22020 At4g22710

EMBL accession:

ED594167

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.