NASC

NASC ID: N566490

Name: SALK_066490

ABRC stock number: SALK_066490

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At5g20250 F5O24.1 40 Exon F5O24.1 40 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_066490.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G20250.4 (at chr 5 pos 6834868 (W/6834805-6834868) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 38, R 37, C 35 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426621.

SALKseq_066490.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G07010.1=P355 and AT3G07020.1=UTR3 (at chr 3 pos 2217389 (C/2217389-2217467) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 38, R 37, C 35 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419988.

SALKseq_066490.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G04760.1 (at chr 4 pos 2424697 (W/2424645-2424697) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 45-71 bps onto the T-DNA. Pools P 38, R 37, C 35 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423304.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_066490

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662790 (SALK_066490C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G07010 AT3G07020 AT4G04760 At5g20250

EMBL accession:

BZ593202

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.