NASC

NASC ID: N567073

Name: SALK_067073

ABRC stock number: SALK_067073

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_067073.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G21980.1 (at chr 1 pos 7736782 (C/7736782-7736855) on the TAIR10). The sequences' 3-77 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 41, R 46, C 45 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392944.

SALKseq_067073.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G35461.1 and AT1G35465.1=P355 (at chr 1 pos 13045118 (C/13045118-13045194) on the TAIR10). The sequences' 1-77 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 41, R 46, C 45 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386255.

SALKseq_067073.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G28650.1 (at chr 4 pos 14144277 (W/14144213-14144277) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 41, R 46, C 45 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396291.

SALKseq_067073.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G19835.2=P355 and AT1G19840.1 (at chr 1 pos 6866378 (C/6866378-6866453) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 41, R 46, C 45 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389602.

SALKseq_067073.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G58005.2=UTR3 and AT5G58010.1=UTR3 (at chr 5 pos 23483322 (W/23483252-23483322) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 75-92 bps onto the T-DNA. Pools P 41, R 46, C 45 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399628.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_067073

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653273 (SALK_067073C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G19835 AT1G19840 AT1G35461 AT1G35465 AT4G28650 AT5G58005 AT5G58010 At1g21980

EMBL accession:

ED596100

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.